The Platform

The key elements of the antibody generation and characterization pipeline are:

  1. Folded proteins or protein domains used as antigens.
  2. Antibodies are selected from recombinant libraries using phage display.
  3. Alternative antibody sources will be incorporated, but all should yield fully-sequenced recombinant binders.
  4. Validation experiments are based on cell lines exhibiting physiological levels of target protein expression paired with isogenic knockout cells.
  5. Validation includes immunoprecipitation and mass spectrometry (IP/MS) as well as western blotting and immunostaining from the paired cell lysates.

 

ALS-RAP workflow