The Platform
The key elements of the antibody generation and characterization pipeline are:
- Folded proteins or protein domains used as antigens.
- Antibodies are selected from recombinant libraries using phage display.
- Alternative antibody sources will be incorporated, but all should yield fully-sequenced recombinant binders.
- Validation experiments are based on cell lines exhibiting physiological levels of target protein expression paired with isogenic knockout cells.
- Validation includes immunoprecipitation and mass spectrometry (IP/MS) as well as western blotting and immunostaining from the paired cell lysates.